Coding

Part:BBa_K1061006:Design

Designed by: mengyi sun   Group: iGEM13_SYSU-China   (2013-09-12)


Bax mutant form


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although it is not toxic to most cancer cells, unfortunately, it is toxic to normal cells. So it is not suggested to use it in the design of mammalian circuit. But it will be useful in constructing yeast circuit as we mentioned.


Source

We clone it from the plasmid provided by addgene.

References